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2 edition of Studies on the Escherichia coli transcription activator protein MelR found in the catalog.

Studies on the Escherichia coli transcription activator protein MelR

Jacqueline Claire Williams

Studies on the Escherichia coli transcription activator protein MelR

by Jacqueline Claire Williams

  • 334 Want to read
  • 13 Currently reading

Published by University of Birmingham in Birmingham .
Written in English


Edition Notes

Thesis (Ph.D) - University of Birmingham, School of Biochemistry,1994.

Statementby Jacqueline Claire Williams.
ID Numbers
Open LibraryOL20915491M

To quantitatively study the mechanism of transcription initiation by RNAP, we developed a single-round quenched kinetics assay (Fig. 1) to probe the kinetics of transcription by directly counting the number of transcripts produced over time. Using this assay, we examined the kinetics of E. coli RNAP transcription from distinct RP ITCCited by: 3. characterized in Escherichia coli. Transcription of the E. coli biotin (bio) operon is directly regulated by the biotin-protein ligase, BirA, the enzyme that covalently attaches biotin to its cognate acceptor proteins. Binding of BirA to the bio operator requires dimerization of the protein, which is triggered by BirA-catalyzed synthesis of.

Stringent control of ribosomal protein gene expression in , PNAS Oct71(10) PubMed ID , , Method (Primary source 1) Bacterial protein was first uniformly pre-labelled with [14C]leucine. Regulation of Gene Expression in Escherichia Coli Softcover reprint of the original 1st ed. Edition by E. C. C. Lin (Author), A. Simon Lynch (Author) ISBN ISBN Why is ISBN important? ISBN. This bar-code number lets you verify that you're getting exactly the right version or edition of a book. Format: Paperback.

Backtracked and paused transcription initiation intermediate of Escherichia coli RNA polymerase Eitan Lerner a,1, SangYoon Chung a,1, Benjamin L. Allen b,1, . Select 'Proteomes', type Escherichia coli and click on the looking search icon (Figure 61). Figure Proteomes search for 'Escherichia Coli'. You get a results page with 'Escherichia coli (strain K12)' being the top hit. You can also see an icon next to the name showing that this is a Reference Proteome.


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Studies on the Escherichia coli transcription activator protein MelR by Jacqueline Claire Williams Download PDF EPUB FB2

INTRODUCTION. The MelR protein of Escherichia coli is a transcription activator required for the metabolism of the disaccharide melibiose ().MelR activates expression of the melA and melB genes, encoding an α-galactosidase and a melibiose permease, respectively.

These genes are co-transcribed from a single promoter, pmelAB, which is bp from the divergent melR promoter, Cited by:   Caswell R, Webster C, Busby S.

Studies on the binding of the Escherichia coli MelR transcription activator protein to operator sequences at the MelAB promoter. Biochem J. Oct 15; (Pt 2)– [PMC free article] Ebright RH. Identification of amino acid-base pair contacts by genetic methods. Methods Enzymol.

; – transcription units, and further studies are still required. In the current study, the aim was to directly correlate RNAP gene transcription with the strength of core promoter elements. To measure the RNAP binding to any DNA segment in Escherichia coli in vivo, I employed the direct method of chromatinFile Size: 2MB.

Example. The catabolite activator protein (CAP; also known as cAMP receptor protein, CRP) activates transcription at the lac operon of the bacterium Escherichia coli. Cyclic adenosine monophosphate (cAMP) is produced during glucose starvation, binds to CAP, causes a conformational change that allows CAP to bind to a DNA site located adjacent to the lac promoter.

Introduction. The Escherichia coli melibiose (mel) operon encodes proteins that facilitate the metabolism of sion of the mel operon is controlled by a single promoter (the melAB promoter), the expression of which is co‐dependent on two transcription activators, MelR and the cyclic AMP receptor protein (CRP) (Webster et al., ; Belyaeva et al., ).

The Escherichia coli MelR protein is a transcription activator that, in the presence of melibiose, activates expression of the melAB operon by binding to four sites located just upstream of the.

Abstract. The Escherichia coli MelR protein is a transcription activator that autoregulates its own promoter by repressing transcription initiation. Optimal repression requires MelR binding to a site that overlaps the melR transcription start point and to upstream sites.

In this work, we have investigated the different determinants needed for optimal repression and their spatial by: Gene. 68 () Elsevier GEN Transcription from the Escherichia coli melR promoter is dependent on the cyclic AMP receptor protein (Recombinant DNA; transcript mapping; deletion analysis; nucleotide sequence homologies; CRP binding site; DNase footprinting; melAB promoter activity) Christine Webster, Kevin Gaston and Stephen Busby Department of Biochemistry, University Cited by:   Eiji Tamai, Tamara A.

Belyaeva, Stephen J. Busby, Tomofusa Tsuchiya. Welcome to TEC. The bacterial genome is transcribed by a single species of DNA-dependent RNA polymerase (RNAP).

The gene selectivity of RNAP is, however, modulated after interaction with two groups of the regulatory protein, the sigma factor with promoter recognition activity and the transcription factor (TF) that controls the activity and specificity of RNAP holoenzyme.

The Escherichia coli SeqA protein contributes to regulation of chromosome replication by preventing re-initiation at newly replicated origins. SeqA protein binds to new DNA which is hemimethylated at the adenine of GATC sequences.

Most of the cellular SeqA is Cited by: Genome and genetics. The genome. Linkage map of Escherichia coli k edition 7. Linkage of Salmonella typhimurium. Gene-protein index of Escherichia coli K, edition 2. Genome organization.

Locations of native insertion sequence elements. Selectable phenotypes. Alterations in the genome. Analysis of mutagenesis. General recombination in Escherichia coli. Results: Escherichia coli is taken into account as the easiest, quickest, and cheapest host with a fully known genome.

Thus, numerous modifications have been carried out on Escherichia coli to optimize it as a good candidate for protein expression and; as a result, several engineered strains of Escherichia coli have been designed.

In general Cited by: 2. Accurate interpretation of quantitative PCR (qPCR) data requires normalization using constitutively expressed reference genes. Ribosomal RNA is often used as a reference gene for transcriptional studies in E. coli. However, the choice of reliable reference genes has not been systematically validated.

The objective of this study is to identify a set of reliable reference genes for Cited by: More than 30 genes in Escherichia coli including phoA, phoE, pstS, and ugpB, which are related to the transport and assimilation of phosphate, belong to the phosphate (pho) regulon.

These genes are induced by phosphate starvation and require PhoB protein for their transcrip- tion. This chapter describes several of the most common nucleic acid analyses performed in vitro to characterize a cloned DNA segment carrying a gene or genes.

Newer polymerases that are used for DNA sequencing include modified T7 phagederived DNA polymerase and a variety of thermostable DNA polymerases such as that from the thermophilic bacterium Thermus : William Hendrickson, Don Walthers.

Helen J. Wing, Jeff Green, John R. Guest, Stephen J. Busby. Determination of the intracellular concentration of the export chaperone SecB in Escherichia coli.

PLos One. DOI: / Type: Book Chapters Status: Published Year Published: Citation: Crane JM, Randall LL. The Sec System: Protein Export in Escherichia coli. EcoSal Plus. DOI: / Previous article in issue: Osmostress-induced changes in yeast gene expression. Previous article in issue: Osmostress-induced changes in yeast gene expression Next article in issue: Disulphide bridge formation in the periplasm of Escherichia coli: β-lactamase::human lgG3 hinge fusions as a model system.

Here we demonstrate that the Escherichia coli ω protein, which copurifies with RNA polymerase, can function as a transcriptional activator when linked covalently to a DNA-binding protein. We show further that ω can function as an activation target when this covalent linkage is replaced by a pair of interacting polypeptides fused to the DNA.

The first edition of TEC (Transcription Profiling of Escherichia coli) database provides transcription factor (TF) binding sites and intensities genomewidely determined for a total of transcription factors (TFs) under a total of conditions as identified by SELEX-chip screening as noted in “About TEC”.

This TEC database can be used freely, but we kindly request to cite TEC in your.Summary. Previous studies on two Escherichia coli rpoB mutants, carrying single amino acid substitutions at approximate amino acid positions and in the β subunit, showed that these alterations in the RNA polymerase resulted in an apparent reduced response to valine-induced amino acid starvation in vivo and prevented ppGpp-mediated inhibition of transcriptional initiation at stable Cited by: This review provides a brief review of the current understanding of the structure-function relationship of the Escherichia coli nucleoid developed after the overview by Pettijohn focusing on the physical properties of nucleoids.

Isolation of nucleoids requires suppression of DNA expansion by various procedures. The ability to control the expansion of nucleoids in vitro has led to purification Cited by: